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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS

Ainura Adilkhankyzy 1 , Bakhytzhan Duissembekov 2

1 NJSC «Kazakh National Agrarian Research University» ; 2 «Zh. Zhiembayev Kazakh Research Institute of Plant Protection and Quarantine»

doi.org/10.37884/3-2024/21 pp. 187-197 Admitted 24.07.2024 Published 30.09.2024

Abstract

Under laboratory conditions, the cultural, morphological and biochemical properties of strains belonging to the Bacillus thuringiensis group were studied, with their deeper selection, promising as producers of biological products with entomocidal action against harmful organisms. According to the results of serological identification, the isolated bacteria were assigned to three serotypes: 3a3b3c – subspecies Bt kurstaki; H4ab-subspecies Bt sotto and 31 serotypes of the species Bt toguchini. According to the conducted analyses, we found that the studied bacteria in nature are most often found 3a 3b 3c serotypes belonging to the subspecies Bacillus thuringiensis kurstaki (84%). Currently, this subspecies is most widely used as the basis of bacterial biologics.

At the same time, the growth of microorganisms on different nutrient media was studied. According to the results of our research, medium "A" turned out to be more favorable for strains of the bacterium B. thuringiensis, since the components contained in this medium provide the nutritional needs of this culture. This medium was used as a working medium for the isolation of bacteria from natural substrates, during further replanting, as well as during the storage of bacteria for a long time.

Bacillus thuringiensis, strain, entomopathogen, nutrient medium, morphology, biochemical properties, cultural characteristics

01 Introduction

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02 References

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Citation Links

[1]2024. DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS. Izdenister natigeler. 3(103) (Sep. 2024), 187–197. DOI:https://doi.org/10.37884/3-2024/21.
(1)DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS. Izdenister natigeler 2024, No. 3(103), 187-197. https://doi.org/10.37884/3-2024/21.
DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS. (2024). Izdenister Natigeler, 3(103), 187-197. https://doi.org/10.37884/3-2024/21
DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS. Izdenister natigeler, [S. l.], n. 3(103), p. 187–197, 2024. DOI: 10.37884/3-2024/21. Disponível em: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/673. Acesso em: 15 sep. 2026.
“DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS”. 2024. Izdenister Natigeler, no. 3(103) (September): 187-97. https://doi.org/10.37884/3-2024/21.
“DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS” (2024) Izdenister natigeler, (3(103), pp. 187–197. doi:10.37884/3-2024/21.
[1]“DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS”, Izdenister natigeler, no. 3(103), pp. 187–197, Sep. 2024, doi: 10.37884/3-2024/21.
“DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS”. Izdenister Natigeler, no. 3(103), Sept. 2024, pp. 187-9, https://doi.org/10.37884/3-2024/21.
“DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS”. Izdenister natigeler, no. 3(103) (September 30, 2024): 187–197. Accessed September 15, 2026. https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/673.
1.DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS. Izdenister natigeler [Internet]. 2024 Sep. 30 [cited 2026 Sep. 15];(3(103):187-9. Available from: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/673
1.DETERMINATION OF CULTURAL-MORPHOLOGICAL AND BIOCHEMICAL PROPERTIES OF BACILLUS THURINGIENSIS STRAINS. Izdenister natigeler. 2024;(3(103):187-197. doi:10.37884/3-2024/21