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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK)

Oryngul Mukhametzhanova 1 , Ardak Kozhabekova 1 , Kuralay Mazarzhanova 2 , Nurzhan Mukhamadiev 3 , Zhanylkhan Bukabayeva 4

1 Kazakh National Agrarian Research University; 2 S. Seifullin Kazakh Agrotechnical Research University, Astana, Kazakhstan; 3 LLP “Kazakh Research Institute of Plant Protection and Quarantine named after Zh. Zhiembayev”; 4 Alikhan Bokeikhan University

doi.org/10.37884/3-2025/29 pp. 304-313 Admitted 17.07.2025 Published 30.09.2025

Abstract

This study presents the results of research on the acclimatization of Populus diverisifolia Schrenk seedlings obtained through in vitro microclonal propagation. After transferring the plants from sterile culture to greenhouse conditions, the effects of various substrates on growth parameters, survival rate, and root system development were examined. Substrates included mixtures of peat with sand, vermiculite, perlite, and forest soil. The best results were observed with a 1:1 mixture of forest soil and sand, which provided a growth increase of up to 4.5 ± 0.3 cm over 8 weeks and a survival rate of up to 92%. This effectiveness is attributed to the high biological activity and balanced mineral composition of forest soil. Root system development showed a steady progression over the 8-week acclimatization period, with root length reaching 5.8 ± 0.5 cm and the number of lateral roots increasing to 8–12. The obtained data confirm the effectiveness of the selected conditions for microclonal propagation and acclimatization of Populus diverisifolia Schrenk, which holds practical significance for the restoration of tugai forests and the conservation of biodiversity. The study also demonstrates the promising potential of applying biotechnological methods in forest management in Kazakhstan.

Populus diverisifolia schrenk in vitro culture ex vitro acclimatization substrate growth parameters seedling survival root system development

01 Introduction

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02 References

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Citation Links

[1]2025. OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK). Izdenister natigeler. 3 (107) (Sep. 2025), 304–313. DOI:https://doi.org/10.37884/3-2025/29.
(1)OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK). Izdenister natigeler 2025, No. 3 (107), 304-313. https://doi.org/10.37884/3-2025/29.
OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK). (2025). Izdenister Natigeler, 3 (107), 304-313. https://doi.org/10.37884/3-2025/29
OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK). Izdenister natigeler, [S. l.], n. 3 (107), p. 304–313, 2025. DOI: 10.37884/3-2025/29. Disponível em: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/1053. Acesso em: 15 sep. 2026.
“OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK)”. 2025. Izdenister Natigeler, no. 3 (107) (September): 304-13. https://doi.org/10.37884/3-2025/29.
“OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK)” (2025) Izdenister natigeler, (3 (107), pp. 304–313. doi:10.37884/3-2025/29.
[1]“OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK)”, Izdenister natigeler, no. 3 (107), pp. 304–313, Sep. 2025, doi: 10.37884/3-2025/29.
“OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK)”. Izdenister Natigeler, no. 3 (107), Sept. 2025, pp. 304-13, https://doi.org/10.37884/3-2025/29.
“OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK)”. Izdenister natigeler, no. 3 (107) (September 30, 2025): 304–313. Accessed September 15, 2026. https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/1053.
1.OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK). Izdenister natigeler [Internet]. 2025 Sep. 30 [cited 2026 Sep. 15];(3 (107):304-13. Available from: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/1053
1.OPTIMIZATION OF PROPAGATION AND ACCLIMATIZATION CONDITIONS FOR SEEDLINGS OF THE DIVERSIFOLIATE POPLAR (POPULUS DIVERISIFOLIA SCHRENK SCHRENK). Izdenister natigeler. 2025;(3 (107):304-313. doi:10.37884/3-2025/29