Abstract
Highly competitive soybean lines of the variety testing nursery of Kazakh Research Institute of Agriculture and Crop Production LLP, the department of oilseeds and the laboratory of molecular biology located in the south-east of Kazakhstan, as well as the TI3 locus with a zero allele, were obtained as the starting material. The main objective of the study is to reduce the activity of the trypsin inhibitor (TI), one of the factors affecting the nutrients contained in its seeds, when using soy culture. That is, it is important to control the function of an enzyme that reduces the activity of trypsin, which breaks down protein during digestion. Currently, it is impossible to determine such enzymes by the traditional method of selection. In this regard, in our study using molecular biology methods in laboratory conditions, the inhibitory activity of trypsin in soybean line seeds and the close relationship of alleles of the Lox2 lipoxygenase locus using the SSR marker in the Satt 656 primer were revealed. The protein storage spectrum and lipoxygenesis activity in seeds of lines were analyzed B-47/411; B-40/62-24; ZR 107/3; B47/53; Z 8/2; E 12/2; W 8/2; W 8/4; W13/2; IT 1/6; IT 1/5; IT 17/3; Z 40/; IT 1/8; A8/22; IT 24/4; IT 1/7; IT 24/2; IT 1/9, IT1/3; CT-41/1; And-23/7. As a result, a low content of the enzyme against nutrients was obtained in the seeds of B-47/411, IT 1/3, Zh 8/2, b47/53 soybean lines. These lines are recommended for use as a starting material in soybean breeding with a decrease in the content of anti-nutrients in seeds.
01 Introduction
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02 References
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