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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9

Akbota Rakhatkyzy 1 , Laura Yerbolova 1 , Karlygash Aubakirova 1 , Zhibek Bakytzhanova 1 , Nurbol Galiakparov 1

1 M.A.Aitkhozhin Institute of Molecular Biology and Biochemistry

doi.org/10.37884/4-2024/19 pp. 192-200 Admitted 22.10.2024 Published 30.12.2024

Abstract

The apple tree M9 is the most promising species for horticulture in the southern part of Western Europe and Kazakhstan, but it is difficult to propagate in in vitro. Apical and axillary segments of the bud measuring 0.5–2.0 cm were used as explants. Microcloning was carried out by adding 6-benzylaminopurine (BAP) – 0.5 mg/l; indole-3-butyric acid (IBA) and gibberellic acid (GA) – 0.5 mg/l to unmodified Murashige-Skoog (MS) and Woody plant medium (WPM) nutrient media. The vitamins B1 and B6 were also added in the amount of 0.5 mg/l, ascorbic acid in the amount of 1.5 mg/l. The modified nutrient media Murashige-Skoog (MS) and Woody plant medium (WPM) included indole-3-butyric acid (IBA) and gibberellic acid (GA) - 0.01 mg / l; vitamins B1 and B6 were in concentrations of 0.1 mg / l and ascorbic acid 1.0 mg / l. The introduction into new nutrient media was carried out after 4 weeks. The experiments consisted of 25-30 explants and were cultivated in 3 replicates.

The most optimal type of explant were apical shoots. Accordingly, their regeneration level was 40% after 1 and 2 months of transplantation in unmodified nutrient media Murashige-Skoog (MS) and Woody plant medium (WPM). Also, the amount of vitamins 0.5 mg / l led to the death of explants after 2-3 weeks. Microcuttings formed callus tissue and underwent necrosis 3-4 months after transplantation. The use of 0.5 mg/l in modified Murashige-Skoog medium, indole-3-butyric acid (IBA) and gibberellic acid (GA) – 0.01 mg/l, vitamins B1 and B6 0.1 mg/l and ascorbic acid 1.0 mg/l completely solved the problem of intensive callus formation. After 1 month of cultivation in this medium, the regenerative capacity of explants was 40.7% higher (65.0-85.0%, respectively) compared to other media. 

micropropagation, in vitro, clonal rootstock, apple, callus, morphogenesis, regeneration.

01 Introduction

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02 References

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Citation Links

[1]2024. MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9. Izdenister natigeler. 4 (104) (Dec. 2024), 192–200. DOI:https://doi.org/10.37884/4-2024/19.
(1)MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9. Izdenister natigeler 2024, No. 4 (104), 192-200. https://doi.org/10.37884/4-2024/19.
MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9. (2024). Izdenister Natigeler, 4 (104), 192-200. https://doi.org/10.37884/4-2024/19
MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9. Izdenister natigeler, [S. l.], n. 4 (104), p. 192–200, 2024. DOI: 10.37884/4-2024/19. Disponível em: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/748. Acesso em: 15 sep. 2026.
“MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9”. 2024. Izdenister Natigeler, no. 4 (104) (December): 192-200. https://doi.org/10.37884/4-2024/19.
“MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9” (2024) Izdenister natigeler, (4 (104), pp. 192–200. doi:10.37884/4-2024/19.
[1]“MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9”, Izdenister natigeler, no. 4 (104), pp. 192–200, Dec. 2024, doi: 10.37884/4-2024/19.
“MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9”. Izdenister Natigeler, no. 4 (104), Dec. 2024, pp. 192-00, https://doi.org/10.37884/4-2024/19.
“MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9”. Izdenister natigeler, no. 4 (104) (December 30, 2024): 192–200. Accessed September 15, 2026. https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/748.
1.MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9. Izdenister natigeler [Internet]. 2024 Dec. 30 [cited 2026 Sep. 15];(4 (104):192-200. Available from: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/748
1.MICROCLONAL PROPAGATION OF THE CLONAL ROOTSTOCK M9. Izdenister natigeler. 2024;(4 (104):192-200. doi:10.37884/4-2024/19