Abstract
This article presents the results of the introduction of embryo transplantation technology at the breeding farm of Baisserke-Agro LLP in the Talgar district of the Almaty region, where the technology of transvaginal aspiration of donor oocytes in cows and their extracorporeal fertilization has been optimized.
The results of our experiments show that the technology of obtaining embryos from highly productive animals using transvaginal aspiration of oocytes and their extracorporeal fertilization is an acceptable method of obtaining offspring with high genetic potential. In our experiments, the yield of suitable oocytes per animal ranged from 3 to 6 oocytes. The optimal follicle diameter recommended for puncture is 3-4 mm or more, and the fertilization rate of oocytes in vitro ranged from 60% to 83.3%.
We also performed ultrasound scanning of the ovaries of donor cows, assessed the functional state of the corpus luteum in recipients by transrectal palpation and ultrasound examination, selected recipients based on rectal palpation and scanning of the corpus luteum of the ovary, recording donor and recipient cows, monitoring recipient animals, and mastering the technology of transvaginal oocyte aspiration.
The introduction of innovative biotechnological reproduction techniques (embryo transplantation, transvaginal aspiration of oocytes) intensifies the selection process in dairy cattle breeding and allows for the modeling of herd structure and an increase in the number of female animals.
01 Introduction
The full text of the article is available for download in PDF format on the right panel.
02 References
- 1 Wathes D, Taylor V, Cheng Z, Mann G, 2003. Follicle growth, corpus luteum function and their effects on embryo development in postpartum dairy cows. Reproduction supplement 61: 216-237.
- 2 M. C. Lucy. The bovine dominant ovarian follicle. J. Anim. Sci. 2007. 85( E. Suppl.):E89–E99
- 3 Ussenbekov Y., Kuzmina T., Boytseva E., Denisenko V. The joint treatment of sperm by prolactin and GTP have determined the increase of the number acrosome-reacted spermatozoa in bulls.31st Scientific meeting of the AETE in Ghent (Belgium) on 11-12th of SeptemberP 200
- 4 Reuben J. MAPLETOFT, Kristina BENNETT STEWARD, Gregg P. ADAMS. Recent advances in the superovulation in cattle. Reprod. Nutr. Dev. 42 (2002) 601–611
- 5 R.J. Mapletoft, , G.A. Bó. Innovative strategies for superovulation in cattle. Anim. Reprod., v.10, n.3, p.174-179, Jul./Sept. 2013
- 6 M. Sakatani, N. V. Alvarez, M. Takahashi, and P. J. Hansen. Consequences of physiological heat shock beginning at the zygote stage on embryonic development and expression of stress response genes in cattle. Journal of Dairy Science Vol. 95 No. 6, 2012 P 3080–3091
- 7 R. Boni. Ovum pick-up in cattle: a 25 yr retrospective analysis . Anim Reprod, v.9, n.3, p.362-369, Jul./Sept. 2012
- 8 D.V. PATEL, N.N. CHAUDHARI, S.R. PATIL, J.G. BHATOL, S.D. BHALODIA AND C.P. DEVANAND. ULTRASONOGRAPHIC ELECTIVE SUPEROVULATION AND ITS RESPONSES IN CROSSBRED AND ZEBU CATTLE. Indian Journal of Animal Reproduction 34 (1) : June 2013 P 17-21
- 9 J.H.M. Viana, E.K.N. Arashiro, L.G.B. Siqueira1, A.M. Ghetti, V.S. Areas, C.R.B. Guimarães, M.P. Palhao, L.S.A. Camargo, C.A.C. Fernandes. Doppler ultrasonography as a tool for ovarian management.Anim. Reprod., v.10, n.3, p.215-222, Jul./Sept. 2013
- 10 JJ.Ireland, F.Ward, F.Jimenez-Krassel, J.L.H.Ireland, G.W.Smith, P.Lonergan and A.C.O.Evans. Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle. Human Reproduction Vol.22, No.6 pp. 1687–1695, 2007
- 11 Andreas Vernunft, Mona Schwerhoff, Torsten Viergutz, Mike Diederich and Andreas Kuwer. 2015 Anti-Muellerian hormone l evels in plasma of Holstein-Friesian heifersas a predictive parameter for ovum pick-up and embryo productionoutcomes. Journal of Reproduction and Development, Vol. 61, No 1, page 74-79