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STOCK-RAISING AND VETERINARY

STUDY OF POLYMORPHISM IN THE mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN

Rauan Amzeev , Marina Yermekova 1 , Tіlek Kapassuly 1 , Makpal Amandykova 2 , Kairat Dossybayev 1 , Tulendy Nurkenov 3

1 LLP “Kazakh Research Institute of Livestock and Foraging”; 2 RSE “Institute of Genetics and Physiology” SC MSHE RK; 3 Nurkenov Tulendy

doi.org/10.37884/3-2025/03 pp. 23-33 Admitted 12.07.2025 Published 30.09.2025

Abstract

In the course of the study, an analysis was conducted to determine the genetic diversity of the fat-tailed coarse-wool Gissar sheep bred in Kazakhstan using SNP polymorphism in the mitochondrial DNA d-loop region, which consists of an 840 base pair (bp) sequence. Blood and wool follicle samples were collected from Gissar sheep, and 30 animals were selected from the population. DNA molecules were extracted from the samples, and PCR was performed using appropriate primers. The amplified product was then subjected to sequencing. As a result, the nucleotide sequence was found to consist of 33.46% adenine, 34.19% thymine, 13.42% guanine, and 18.93% cytosine. The sequence analysis of the Gissar sheep was aligned with sequence data of domestic and wild sheep breeds available in the NCBI database using the BLAST program. A phylogenetic analysis conducted using the MEGA X program revealed a high level of genetic relatedness (74–80%) among Kazakh Hisar sheep. The Hisar breed originated from the same branch as the Duamo and Khaikha sheep, showing a close phylogenetic relationship. In contrast, European and some Asian sheep breeds were found to be genetically distant, indicating that they belong to different evolutionary lineages.

genetic diversity d-loop genotype DNA markers phylognetics sequencing haplotope

01 Introduction

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02 References

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Citation Links

[1]2025. STUDY OF POLYMORPHISM IN THE mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN. Izdenister natigeler. 3 (107) (Sep. 2025), 23–33. DOI:https://doi.org/10.37884/3-2025/03.
(1)STUDY OF POLYMORPHISM IN THE Mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN. Izdenister natigeler 2025, No. 3 (107), 23-33. https://doi.org/10.37884/3-2025/03.
STUDY OF POLYMORPHISM IN THE mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN. (2025). Izdenister Natigeler, 3 (107), 23-33. https://doi.org/10.37884/3-2025/03
STUDY OF POLYMORPHISM IN THE mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN. Izdenister natigeler, [S. l.], n. 3 (107), p. 23–33, 2025. DOI: 10.37884/3-2025/03. Disponível em: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/995. Acesso em: 15 sep. 2026.
“STUDY OF POLYMORPHISM IN THE Mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN”. 2025. Izdenister Natigeler, no. 3 (107) (September): 23-33. https://doi.org/10.37884/3-2025/03.
“STUDY OF POLYMORPHISM IN THE mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN” (2025) Izdenister natigeler, (3 (107), pp. 23–33. doi:10.37884/3-2025/03.
[1]“STUDY OF POLYMORPHISM IN THE mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN”, Izdenister natigeler, no. 3 (107), pp. 23–33, Sep. 2025, doi: 10.37884/3-2025/03.
“STUDY OF POLYMORPHISM IN THE Mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN”. Izdenister Natigeler, no. 3 (107), Sept. 2025, pp. 23-33, https://doi.org/10.37884/3-2025/03.
“STUDY OF POLYMORPHISM IN THE Mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN”. Izdenister natigeler, no. 3 (107) (September 30, 2025): 23–33. Accessed September 15, 2026. https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/995.
1.STUDY OF POLYMORPHISM IN THE mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN. Izdenister natigeler [Internet]. 2025 Sep. 30 [cited 2026 Sep. 15];(3 (107):23-3. Available from: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/995
1.STUDY OF POLYMORPHISM IN THE mt DNA D-LOOP REGION OF THE GISSAR SHEEP BREED RAISED IN KAZAKHSTAN. Izdenister natigeler. 2025;(3 (107):23-33. doi:10.37884/3-2025/03