Menu
AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS

Svetlana Dolgikh 1 , Balnur Kabylbekova 2 , Sagi Soltanbekov 3 , Akerke Tashkenbayeva 2 , Moldir Sarshayeva 3 , Zarina Yusupova 4

1 LLP "Kazakh Fruit and Vegetable Research Institute"; 2 Kazakh Fruit and Vegetable Research Institute; 3 Kazakh Scientific Research Institute of Fruit and Vegetable Growing; 4 Kazakh Research Institute of Fruit and Vegetable Growing

doi.org/10.37884/2-2025/22 pp. 223-233 Admitted 03.04.2025 Published 30.06.2025

Abstract

Modern horticulture requires seedlings with low growth and compact crowns, virus-free and of high quality, which ensures the intensity and high productivity of the garden. The method of clonal micropropagation has a significant place in the system of virus-free planting material production. The use of biotechnological methods in nursery production allows increasing the efficiency of plant health improvement up to 100%, 5-10 times and more increase the multiplication factor and 2-3 years accelerate the introduction of new healthy varieties and forms into production. The presented studies determined the composition of viral diseases in mother plants of apple rootstocks and optimized the stages of clonal micropropagation with modification of nutrient medium composition at the stage of rhizogenesis, as well as their adaptation to transfer to in vivo conditions. As a result of work in container culture, 126 base plants of Arm 18 and 31 base plants of Zhetysu-5 rootstock were obtained by cuttings of virus-free pre-basic mother plants.

clonal rootstocks apple viruses biotechnology in vitro micropropagation nutrient medium virus-free material

01 Introduction

The full text of the article is available for download in PDF format on the right panel.

02 References

  1. 1 Rodríguez-Verástegui L. L. et al. Viruses infecting trees and herbs that produce edible fleshy fruits with a prominent value in the global market: An evolutionary perspective //Plants. – 2022. – Т. 11. – №. 2. – С. 203.
  2. 2 Li Y. et al. Characterization of apple stem grooving virus and apple chlorotic leaf spot virus identified in a crab apple tree //Archives of virology. – 2017. – Т. 162. – №. 4. – С. 1093-1097.
  3. 3 Kerimbek N. et al. Assessment of occurrence and diversity of apple viruses in south kazakhstan //Ġylym ža̋ne bìlìm. – 2024. – Т. 1. – №. 2 (75). – С. 79-85.
  4. 4 Hu G. et al. Virus elimination from in vitro apple by thermotherapy combined with chemotherapy //Plant Cell, Tissue and Organ Culture (PCTOC). – 2015. – Т. 121. – С. 435-443.
  5. 5 Tan R. et al. Enhanced efficiency of virus eradication following thermotherapy of shoot-tip cultures of pear //Plant Cell, Tissue and Organ Culture (PCTOC). – 2010. – Т. 101. – С. 229-235.
  6. 6 Wang L.P. et al. Effect of thermotherapy on elimination of Apple stem grooving virus and Apple chlorate leaf spot virus for in vitro cultured pear shoot tips//Hort.Science. – 2006. – T.41. – P.729-732.
  7. 7 Laimer M., Barba M. Elimination of systemic pathogens by thermotherapy, tissue culture, or in vitro micrografting //Virus and virus-like diseases of pome and stone fruits. – 2011. – С. 389-393.
  8. 8 Panattoni A., Luvisi A., Triolo E. Elimination of viruses in plants: twenty years of progress //Spanish journal of agricultural research. – 2013. – Т. 11. – №. 1. – С. 173-188.
  9. 9 Smith G. R. et al. Plant pathogen eradication: determinants of successful programs //Australasian Plant Pathology. – 2017. – Т. 46. – №. 3. – С. 277-284.
  10. 10 Кухарчик Н.В., Кастрицкая М.С., Колбанова Е.В. и др.Размножение плодовых, ягодных растений, винограда и хмеля в культуре тканей in vitroю- Минск.- «Колорград».- 2021.-397 с.
  11. 11 Митрофанова О.В., Лесникова-Седошенко Н.П., Ходаков Г.В. Биотехнология в селекции и оздоровлении косточковых плодовых и субтропических культур / Фактори эксппериментальной эволюции организмов: Зб. наук. пр. Укр. т-ва генетикови селекционеров И.Вавилова /За ред. Ь.И. Ройка. – К.: Логос, 2006. –Е.3. –С.619-624
  12. 12 Barba M. et al. Viroid elimination by thermotherapy, cold therapy, tissue culture, in vitro micrografting, or cryotherapy //Viroids and Satellites. – Academic Press, 2017. – С. 425-435.
  13. 13 Sedlák J., Semerák M., Rejlová M. Sanitation of Apple Cultivars from AP Phytoplasma and ApMV and ACLSV Viruses Using In Vitro Culture and Cryo-Knife Therapy in Liquid Nitrogen //Applied Sciences. – 2023. – Т. 13. – №. 13. – С. 7527.
  14. 14 Sedlák J. et al. Elimination of Solanum nigrum ilarvirus 1 and apple hammerhead viroid from apple cultivars using antivirals ribavirin, rimantadine, and zidovudine //Viruses. – 2023. – Т. 15. – №. 8. – С. 1684.
  15. 15 Wang M. R. et al. In vitro thermotherapy-based methods for plant virus eradication //Plant methods. – 2018. – Т. 14. – С. 1-18.
  16. 16 Огородникова О. В. Биологическое тестирование неповирусов земляники садовой на индикаторном растении Cucumis sativus //Генофонд и селекция растений. – 2020. – С. 78-82.
  17. 17 Колбанова Е.В., Кухнарчик Н.В. Методика микроразмножения плодовых культур in vitro // ж. Плодоводство: науч. тр. ин-т плодоводства НАН Белоруссии. – Самохваловичи, 2006. – т. 18, ч.2 – с.163-168.
  18. 18 Долгих С.Г. Рекомендации «Технология производства безвирусного посадочного материала плодовых, ягодных культур и винограда».- 2020.- 57 с.
  19. 19 Уразаева М., Ефремова Ю., Ормакаев А. Интродуцированные клоновые подвои косточковых //Izdenister natigeler. – 2023. – №. 2 (98). – С. 143-153.
  20. 20 Рахаткызы А. и др. М9 клонды телітушісін микроклонды көбейту //Izdenister natigeler. – 2024. – №. 4 (104). – С. 192-200.

Citation Links

[1]2025. DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. Izdenister natigeler. 2 (106) (Jun. 2025), 223–233. DOI:https://doi.org/10.37884/2-2025/22.
(1)DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. Izdenister natigeler 2025, No. 2 (106), 223-233. https://doi.org/10.37884/2-2025/22.
DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. (2025). Izdenister Natigeler, 2 (106), 223-233. https://doi.org/10.37884/2-2025/22
DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. Izdenister natigeler, [S. l.], n. 2 (106), p. 223–233, 2025. DOI: 10.37884/2-2025/22. Disponível em: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/908. Acesso em: 15 sep. 2026.
“DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS”. 2025. Izdenister Natigeler, no. 2 (106) (June): 223-33. https://doi.org/10.37884/2-2025/22.
“DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS” (2025) Izdenister natigeler, (2 (106), pp. 223–233. doi:10.37884/2-2025/22.
[1]“DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS”, Izdenister natigeler, no. 2 (106), pp. 223–233, Jun. 2025, doi: 10.37884/2-2025/22.
“DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS”. Izdenister Natigeler, no. 2 (106), June 2025, pp. 223-3, https://doi.org/10.37884/2-2025/22.
“DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS”. Izdenister natigeler, no. 2 (106) (June 30, 2025): 223–233. Accessed September 15, 2026. https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/908.
1.DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. Izdenister natigeler [Internet]. 2025 Jun. 30 [cited 2026 Sep. 15];(2 (106):223-3. Available from: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/908
1.DETERMINATION OF THE VIRAL DISEASES COMPOSITION OF STONE FRUIT CLONAL ROOTSTOCKS IN KAZAKHSTAN AND IN VITRO REPRODUCTION FEATURES OF HEALTHY PLANTS. Izdenister natigeler. 2025;(2 (106):223-233. doi:10.37884/2-2025/22