SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE

U.T. Zhumatayeva*; B.A. Duisembekov; A.M. Begalieva; U.O. Sabdenova; K.K. Yerkekulova

doi:10.37884/2-2021/13

Abstract

The article provides a preliminary screening of Asian locust larvae aged 2-3 years for the virulence of 25 isolates isolated from insects belonging to different systematic groups of fungi Beauveria bassiana. Five of the 25 strains showed high biological activity against larvae, (BCo1-14, BSc1-15, BSc2-15, BTr1-16, BPit-16) reaching a mortality rate of 90-100% 21 days after inoculation. The strains that have the best effect on the level and rate of mortality of the host organism are - BCo1-14, BSc1-15, BSc2-15, BTr1-16, BPit-16. The greatest biological activity was observed in the BSc1-15 strain, as the mortality rate of test organisms was only 100% within 15 days of exposure. The lowest activity was shown by the BCi₄-14 strain in the range of 35.0-62.5%. At the same time, the mortality rate of Asian locust larvae in the control variant (without treatment) for 21 days was 15.0±2.88%. Thus, the proportion of highly toxic forms (80-100% lethality) in the total number of studied strains was not more than 44%, and the proportion of mildly toxic forms (less than 80% activity) was 56%.

еntomopathogen, virulent, Beauveria bassiana, strain, crop, Locusta migratoria migratoria L., conidia, fungi, screening.

01 Introduction

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02 References

1. Lachininskiy A.V., Sergeyev M.G., Childebayev M.K., Chernyakhovskiy M.Ye., Lokvud A.Dzh., Kambulin V.Ye., Gapparov F.A. (2002). Saranchovyye Kazakhstana, Sredney Azii i sopredel'nykh territoriy [Locusts of Kazakhstan, Central Asia and adjacent territories]. – SSHA, Larami: Mezhdunarod. assots. prikl. Akridologii i universitet Vayominga, – ss. 387 [in Russian].
2. Kambulin V.Ye., Yskak S., Toleubayev K.M. (2010) Dinamika populyatsiy stadnykh saranchovykh v Kazakhstane [Dynamics of herd locust populations in Kazakhstan] // Zashchita i karantin rasteniy. –– №4. – ss. 17-20[in Russian].
3. Glupova V.V. (2001) Insect pathogens: structural and functional aspects / edited by Glupova V.V. – М7: All year round. - 736 p [in English].
4. Serebrov V.V., Kiselev A.A., Glupov V.V. (2003) Izucheniye nekotorykh faktorov sinergizma mezhdu entomopatogennymi gribami i khimicheskimi insektitsidami [Study of some factors of synergy between entomopathogenic fungi and chemical insecticides]// Mikologiya i fitopatologiya. –T. 37. - V. 1. - ss.76 -81[in Russian].
5. Slyamova N.D., Smagulova Sh.B., Abdukadyrova A.D., Bolatbekova B.K., Uspanov A.M. (2017) Ekologicheski bezopasnyye metody kontrolya chislennosti koloradskogo zhuka s ispol'zovaniyem entomopatogennykh gribov v usloviyakh Yugo-Vostoka Kazakhstana [Environmentally safe methods of population control of the Colorado potato beetle using entomopathogenic fungi in the conditions of the South-East of Kazakhstan]// «Issledovaniya, rezul'taty». Almaty, № 4 (76) ss. 436-442 [in Russian].
6. Smagulova Sh.B., Duysembekov B.A., Slyamova N.D., Uspanov A.M., Lednev G.R., Levchenko M.V. (2017) Entomopatogennyye anamorfnyye askomitsety v populyatsiyakh zhukovkoroyedov v Yugo-Vostochnom Kazakhstane i otsenka ikh spetsifichnosti [Ento-mopathogenic anamorphic ascomycetes in beetle-eating populations in South-Eastern Kazakhstan and assessment of their specificity] // Issledovaniya, rezul'taty. №4 (76) - ss. 449-457 [in Russian].
7. Lukina A.V., Lednev G.R., Duysembekov B.A., Levchenko M.V., Slyamova N.D., Smagulova Sh.B. (2006) Poisk i vydeleniye novykh shtammov entomopatogennykh gribov v yugo-vostochnom Kazakhstane [1. Search and isolation of new strains of entomopathogenic fungi in south-eastern Kazakhstan] // I-ya Mezhd. nauchn. konf. molodykh uchenykh i aspirantov «Aktual'nyye problemy zashchity i karantina rasteniy». – Almaty, – рр. 99-101[in Russian].
8. Abdukerim R.Zh., Tulengutova K.N., Khidirov K.R., Zhunusova A.S., Alimkulova M.K. (2017) Biologicheskaya aktivnost' entomopatogennykh gribov vydelennykh iz koroyeda na nasekomykh iz drugikh sistematicheskikh grupp [Biological activity of entomopathogenic fungi isolated from bark beetles on insects from other systematic groups] // «Issledovaniya, rezul'taty». Almaty, № 4 (76). ss. 222-228[in Russian].
9. Bilay V.I. (1982) (red.) Metody eksperimental'noy mikologii Spravochnik [Methods of experimental mycology]. Kiyev, «Naukova dumka», – ss. 550 [in Russian].
10. Glupov V.V. (2001) Patogeny nasekomykh: strukturnyye i funktsional'nyye aspekty [Insect pathogens: structural and functional aspects]. / Pod.red - M.: Kruglyy god, -ss. 738 [in Russian].
11. Faria, M., Wraight, S.P. Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types. // Biological Control. 2007. - V.43. – Р.237-256 [in English].
12. Cliquet S., Jackson M.A. Comparison of air - drying methods for evaluating the desiccation tolerance of liquid culture - produced blastospores of Paecilomyces fumosoroseus. // World J. Microbiol. Biotech. 1997. - V. 13. - P. 299 - 303[in English].
13. Shternshis M.V., Yermakova N.I., Zurabova E.R., Isangalin F.S. (1990) Metodi-cheskiye rekomendatsii [Methodological recommendations]. - M., –ss. 14[in Russian].
14. Labinskaya A.S. (1963) Prakticheskoye rukovodstvo po mikrobiologicheskim metodam issledovaniya [Practical guide to microbiological research methods]. – M.: Gos. izd.-vo med. lit.-ry., –ss. 463 [in Russian].
15. Geshtovt N.Yu. (2002) Entomopatogennyye griby. Biotekhnologicheskiye aspekty [Entomopathogenic fungi. Biotechnological aspects]/ N.Yu. Geshtovt. - Almaty,– ss. 288 [in Russian].
16. Kryukov V.Yu., Yaroslavtseva O.N., Levchenko M.V., Lednev G.R., Glupov V.V. (2009) Fenotipicheskaya izmenchivost' prirodnykh izolyatov entomopatogennogo griba Beauveria bassiana [Phenotypic variability of natural isolates of the entomopathogenic fungus Beauveria bassiana]. //Mikologiya i fitopatologiya. T. 43. N 6. ss. 514–521[in Russian].

Citation Links

[1]2021. SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE. Izdenister natigeler. 2 (90) (Jun. 2021), 124–135. DOI:https://doi.org/10.37884/2-2021/13.

(1)SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE. Izdenister natigeler 2021, No. 2 (90), 124-135. https://doi.org/10.37884/2-2021/13.

SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE. (2021). Izdenister Natigeler, 2 (90), 124-135. https://doi.org/10.37884/2-2021/13

SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE. Izdenister natigeler, [S. l.], n. 2 (90), p. 124–135, 2021. DOI: 10.37884/2-2021/13. Disponível em: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/42. Acesso em: 15 sep. 2026.

“SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE”. 2021. Izdenister Natigeler, no. 2 (90) (June): 124-35. https://doi.org/10.37884/2-2021/13.

“SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE” (2021) Izdenister natigeler, (2 (90), pp. 124–135. doi:10.37884/2-2021/13.

[1]“SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE”, Izdenister natigeler, no. 2 (90), pp. 124–135, Jun. 2021, doi: 10.37884/2-2021/13.

“SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE”. Izdenister Natigeler, no. 2 (90), June 2021, pp. 124-35, https://doi.org/10.37884/2-2021/13.

“SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE”. Izdenister natigeler, no. 2 (90) (June 30, 2021): 124–135. Accessed September 15, 2026. https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/42.

1.SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE. Izdenister natigeler [Internet]. 2021 Jun. 30 [cited 2026 Sep. 15];(2 (90):124-35. Available from: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/42

1.SCREENING OF COLLECTION STRAINS (COLLECTIONS) OF ENTOMOPATHOGENIC FUNGI AGAINST ASIAN LOCUSTS FOR SIGNS OF VIRULENCE. Izdenister natigeler. 2021;(2 (90):124-135. doi:10.37884/2-2021/13

[1] 1. Lachininskiy A.V., Sergeyev M.G., Childebayev M.K., Chernyakhovskiy M.Ye., Lokvud A.Dzh., Kambulin V.Ye., Gapparov F.A. (2002). Saranchovyye Kazakhstana, Sredney Azii i sopredel'nykh territoriy [Locusts of Kazakhstan, Central Asia and adjacent territories]. – SSHA, Larami: Mezhdunarod. assots. prikl. Akridologii i universitet Vayominga, – ss. 387 [in Russian].

[2] 2. Kambulin V.Ye., Yskak S., Toleubayev K.M. (2010) Dinamika populyatsiy stadnykh saranchovykh v Kazakhstane [Dynamics of herd locust populations in Kazakhstan] // Zashchita i karantin rasteniy. –– №4. – ss. 17-20[in Russian].

[3] 3. Glupova V.V. (2001) Insect pathogens: structural and functional aspects / edited by Glupova V.V. – М7: All year round. - 736 p [in English].

[4] 4. Serebrov V.V., Kiselev A.A., Glupov V.V. (2003) Izucheniye nekotorykh faktorov sinergizma mezhdu entomopatogennymi gribami i khimicheskimi insektitsidami [Study of some factors of synergy between entomopathogenic fungi and chemical insecticides]// Mikologiya i fitopatologiya. –T. 37. - V. 1. - ss.76 -81[in Russian].

[5] 5. Slyamova N.D., Smagulova Sh.B., Abdukadyrova A.D., Bolatbekova B.K., Uspanov A.M. (2017) Ekologicheski bezopasnyye metody kontrolya chislennosti koloradskogo zhuka s ispol'zovaniyem entomopatogennykh gribov v usloviyakh Yugo-Vostoka Kazakhstana [Environmentally safe methods of population control of the Colorado potato beetle using entomopathogenic fungi in the conditions of the South-East of Kazakhstan]// «Issledovaniya, rezul'taty». Almaty, № 4 (76) ss. 436-442 [in Russian].

[6] 6. Smagulova Sh.B., Duysembekov B.A., Slyamova N.D., Uspanov A.M., Lednev G.R., Levchenko M.V. (2017) Entomopatogennyye anamorfnyye askomitsety v populyatsiyakh zhukovkoroyedov v Yugo-Vostochnom Kazakhstane i otsenka ikh spetsifichnosti [Ento-mopathogenic anamorphic ascomycetes in beetle-eating populations in South-Eastern Kazakhstan and assessment of their specificity] // Issledovaniya, rezul'taty. №4 (76) - ss. 449-457 [in Russian].

[7] 7. Lukina A.V., Lednev G.R., Duysembekov B.A., Levchenko M.V., Slyamova N.D., Smagulova Sh.B. (2006) Poisk i vydeleniye novykh shtammov entomopatogennykh gribov v yugo-vostochnom Kazakhstane [1. Search and isolation of new strains of entomopathogenic fungi in south-eastern Kazakhstan] // I-ya Mezhd. nauchn. konf. molodykh uchenykh i aspirantov «Aktual'nyye problemy zashchity i karantina rasteniy». – Almaty, – рр. 99-101[in Russian].

[8] 8. Abdukerim R.Zh., Tulengutova K.N., Khidirov K.R., Zhunusova A.S., Alimkulova M.K. (2017) Biologicheskaya aktivnost' entomopatogennykh gribov vydelennykh iz koroyeda na nasekomykh iz drugikh sistematicheskikh grupp [Biological activity of entomopathogenic fungi isolated from bark beetles on insects from other systematic groups] // «Issledovaniya, rezul'taty». Almaty, № 4 (76). ss. 222-228[in Russian].

[9] 9. Bilay V.I. (1982) (red.) Metody eksperimental'noy mikologii Spravochnik [Methods of experimental mycology]. Kiyev, «Naukova dumka», – ss. 550 [in Russian].

[10] 10. Glupov V.V. (2001) Patogeny nasekomykh: strukturnyye i funktsional'nyye aspekty [Insect pathogens: structural and functional aspects]. / Pod.red - M.: Kruglyy god, -ss. 738 [in Russian].

[11] 11. Faria, M., Wraight, S.P. Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types. // Biological Control. 2007. - V.43. – Р.237-256 [in English].

[12] 12. Cliquet S., Jackson M.A. Comparison of air - drying methods for evaluating the desiccation tolerance of liquid culture - produced blastospores of Paecilomyces fumosoroseus. // World J. Microbiol. Biotech. 1997. - V. 13. - P. 299 - 303[in English].

[13] 13. Shternshis M.V., Yermakova N.I., Zurabova E.R., Isangalin F.S. (1990) Metodi-cheskiye rekomendatsii [Methodological recommendations]. - M., –ss. 14[in Russian].

[14] 14. Labinskaya A.S. (1963) Prakticheskoye rukovodstvo po mikrobiologicheskim metodam issledovaniya [Practical guide to microbiological research methods]. – M.: Gos. izd.-vo med. lit.-ry., –ss. 463 [in Russian].

[15] 15. Geshtovt N.Yu. (2002) Entomopatogennyye griby. Biotekhnologicheskiye aspekty [Entomopathogenic fungi. Biotechnological aspects]/ N.Yu. Geshtovt. - Almaty,– ss. 288 [in Russian].

[16] 16. Kryukov V.Yu., Yaroslavtseva O.N., Levchenko M.V., Lednev G.R., Glupov V.V. (2009) Fenotipicheskaya izmenchivost' prirodnykh izolyatov entomopatogennogo griba Beauveria bassiana [Phenotypic variability of natural isolates of the entomopathogenic fungus Beauveria bassiana]. //Mikologiya i fitopatologiya. T. 43. N 6. ss. 514–521[in Russian].