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AGRICULTURE, AGROCHEMICAL, FEED PRODUCTION, AGROECOLOGY

MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN

Angelina Malysheva 1 , Alma Kokhmetova 1 , Madina Kumarbayeva 1 , Zhenys Keyshilov 1 , Saltanat Dubekova 2

1 Institute of Plant Biology and Biotechnology; 2 Kazakh Research Institute of Agriculture and Plant Growing

doi.org/10.37884/3-2023/09 pp. 92-99 Admitted 03.07.2023 Published 29.09.2023

Abstract

Leaf rust caused by the parasitic basidiomycete Puccinia recondita f.sp. tritici, one of the most harmful diseases of spring soft wheat both in Kazakhstan and in the world. The development of rust diseases can lead to a significant reduction in the yield of susceptible wheat varieties. The main problem of wheat breeding is the loss of efficiency of resistance genes, which is associated with the emergence of new virulent races of pathogens. This article presents the results of assessing the resistance of spring wheat varieties at the stage of seedlings and adult plants, as well as the results of screening for Lr resistance genes using molecular markers. As a result of the experiments, 9 samples were selected, demonstrating a high level of resistance to both races of P. recondita - MKT/Q and TJT/R at the seedling stage. In the field for two years of testing, 6 varieties were selected that showed a high level of resistance to the pathogen. The use of molecular markers made it possible to identify carriers of effective Lr resistance genes. Based on the results of molecular screening, 4 carriers of the Lr9 resistance gene were identified; 5 carriers of the Lr10 gene; 7 carriers Lr26; 7 carriers of Lr34 and 2 carriers of the Lr46 gene. The results obtained can be used in the breeding programs of Kazakhstan.

leaf rust, P. recondita, spring wheat, Lr-genes, virulence, molecular markers, marker associated selection

01 Introduction

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02 References

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Citation Links

[1]2023. MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN. Izdenister natigeler. 3 (99) (Sep. 2023), 92–99. DOI:https://doi.org/10.37884/3-2023/09.
(1)MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN. Izdenister natigeler 2023, No. 3 (99), 92-99. https://doi.org/10.37884/3-2023/09.
MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN. (2023). Izdenister Natigeler, 3 (99), 92-99. https://doi.org/10.37884/3-2023/09
MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN. Izdenister natigeler, [S. l.], n. 3 (99), p. 92–99, 2023. DOI: 10.37884/3-2023/09. Disponível em: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/315. Acesso em: 15 sep. 2026.
“MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN”. 2023. Izdenister Natigeler, no. 3 (99) (September): 92-99. https://doi.org/10.37884/3-2023/09.
“MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN” (2023) Izdenister natigeler, (3 (99), pp. 92–99. doi:10.37884/3-2023/09.
[1]“MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN”, Izdenister natigeler, no. 3 (99), pp. 92–99, Sep. 2023, doi: 10.37884/3-2023/09.
“MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN”. Izdenister Natigeler, no. 3 (99), Sept. 2023, pp. 92-99, https://doi.org/10.37884/3-2023/09.
“MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN”. Izdenister natigeler, no. 3 (99) (September 29, 2023): 92–99. Accessed September 15, 2026. https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/315.
1.MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN. Izdenister natigeler [Internet]. 2023 Sep. 29 [cited 2026 Sep. 15];(3 (99):92-9. Available from: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/315
1.MOLECULAR GENETIC SCREENING OF LR-RESISTANCE GENES IN ACCESSIONS OF SOFT SPRING WHEAT IN THE CONDITIONS OF THE SOUTHEAST OF KAZAKHSTAN. Izdenister natigeler. 2023;(3 (99):92-99. doi:10.37884/3-2023/09