ВЛИЯНИЕ РАЗЛИЧНЫХ СТИМУЛЯТОРОВ НА КАЛЛУСОГЕНЕЗ  JUGNALIS REGIA EFFIGIA

Ельвира Шаденова; Мариям  Кайгермазова; Медет  Сембеков; Умит  Ошакбай; Эрика  Джангалина

doi:10.37884/2-2023/33

Abstract

The sources of phytopreparations are medicinal plants that are valued all over the world and which may disappear in an era of climate change. One such source is the walnut. Walnut is a member of the genus Juglans L. in the family Juglandaceae. The aim of the study is to investigate the effect of different concentrations of growth stimulants on different Juglans regia еffigia explants using microclonal propagation technology, where mature trees were taken as mother plants. This study is designed to produce an additional medicinal source of raw materials in the form of callus or suspension cultures. The best results were achieved when the explants were initiated on DKW culture medium using 1 and 10 mg/l BAP, 5 and 10 mg/l TDZ. After 20 days, the average number of shoots formed was 2-5 per callus, with a shoot height of 1.6-2.7 cm.

microclonal multiplication callus callus genesis explant in vitro walnut tissue culture

01 Introduction

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02 References

1. Буркова Е.А., Канарский А.В., Канарская З.А. (2014). Перспектива применения фитобиотехнологии для получения биологически активных веществ //Вестник Казанского технологического университета, 2014. -Т.14. – С.352–356;
2. Mulabagal V., Tsay H-S. Plant cell cultures -an alternative and efficient source for the production of biologically important secondary metabolites //Int.Journal Applied Science Engineering, 2004. – V.2. - №1. – Р.29-48.
3. Trandafir I., Cosmulescu S., Botu M., Nour V. Antioxidant activity, and phenolic and mineral contents of the walnut kernel (Juglans regia L.) as a function of the pellicle color //Fruits, 2016. -V. 71. – Р.173–184. doi:10.1051/fruits/2016006.115
4. Hojatollah Kavosi Ali Khadivi. The selection of superior late-leafing genotypes of Persian walnut (Juglans regia L.) among seedling originated trees based on pomological characterizations //Scientia Horticulturae, 2021. - V.28. -P. 110-299. DOI: 10.1016/j.scienta.2021.110299.
5. Olimpia A. Iordănescu, Isidora Radulov, Ioana P. Buhan et al. Physical, Nutritional and Functional Properties of Walnuts Genotypes (Juglans regia L.) from Romania //Agronomy, 2021. -V.11. -P.1092. https://doi.org/10.3390/agronomy11061092.
6. Змушко А.А., Рундя А.П. Размножение грецкого ореха (juglans regia l.) in vitro //Плодоводство, 2015. - Т. 27. – С.404-410.
7. Ricardo Julian Licea-Moreno, Alexandru Fira, Georgi Chocov. Micropropagation of valuable walnut genotypes for timber production: new advances and insights //Annals of Silvicultural Research, 2020. 44 (1). -P.5-13. DOI: http://dx.doi.org/10.12899/asr-1932.
8. Кушнаренко С.В., Ромаданова Н.В., Турдиев Т.Т., Аралбаева М.М., Қалыбаев Қ.Р. Сохранение в криобанке образцов грецкого ореха из нескольких популяций сайрам-угамского государственного национального природного парка //Вестник КазНУ. Серия экологическая. -№2. -Т.71. - С.72-80. https://doi.org/10.26577/EJE.2022.v71.i2.07.
9. Kepeneka, K. and Kolağasib, Z. 2016. Micropropagation of walnut (Juglans regia L.). Acta Physica Polonica, Vol. 130, No. 1, 150 – 156.
10. López, J.M. Field behavior of self-rooted walnut trees of different cultivars produced by tissue culture and planted in Murcia (Spain) //Acta Hort., 2001.- 544. -P. 543–546.
11. Manjkhola, S., U. Dhar, and M. Joshi. Organogenesis, embryogenesis and synthetic seed production in Arnebia euchroma an critically endangered medicinal plant of the Himalaya //In Vitro Cell. Dev. Biol.-Plant, 2005. -V.41. – P.244-248.
12. Bandyopadhyay S., Cane K., Rasmussen G., and Hamill J. Efficient plant regeneration from seedling explants of two commercially important temperate eucalypt species–Eucalyptus nitens and E. Globulus //Plant Sci., 2005. – 140. – P.189-198.
13. Xiao-Dong CAI, Gui-Yuan WANG, Wen-Juan CAO. In vitro Induction and Proliferation of Callus from Immature Cotyledons and Embryos of Juglans regia cv. ‘Xiangling’ //NotBotHorti Agrobo, 2013. - 41(2). -P.378-384.
14. Fatima, A., Kamili A.N., and Shah A.M. Plantlet regeneration from excised embryonal axes, shoot apices and nodal segments of Juglans regia L. //Acta Hort., 2006 (ISHS)-705. -P.387-392.
15. Hasey et al., Hasey, J.K., Westerdahl, B.B., Micke, W.C., Ramos, D.E. &Yeater, J.T. Yield performance of own-rooted “Chandler” walnut versus “Chandler” walnut on Paradox Rootstock //Acta Hort., 2001. -P. 489–493.
16. Fernández H., Pérez C., and Sánchez-Tamés R. Modulation of the morphogenic potential of the embryonic axis of Juglans regia by cultural conditions //Plant Growth Regul.,2000. -V. 30. -P.125-131.
17. Vahdati K., Jariteh M., Niknam V., Mirmasoumi M., and Ebrahimzadeh H. Somatic embryogenesis and embryo maturation in Persian walnut //Acta Hort., 2006.- 705. -P.199-205.

Citation Links

[1]2023. EFFECTS OF DIFFERENT STIMULANTS ON CALLUSOGENESIS JUGNALIS REGIA EFFIGIA. Izdenister natigeler. 2 (98) (Jun. 2023), 336–344. DOI:https://doi.org/10.37884/2-2023/33.

(1)EFFECTS OF DIFFERENT STIMULANTS ON CALLUSOGENESIS JUGNALIS REGIA EFFIGIA. Izdenister natigeler 2023, No. 2 (98), 336-344. https://doi.org/10.37884/2-2023/33.

EFFECTS OF DIFFERENT STIMULANTS ON CALLUSOGENESIS JUGNALIS REGIA EFFIGIA. (2023). Izdenister Natigeler, 2 (98), 336-344. https://doi.org/10.37884/2-2023/33

EFFECTS OF DIFFERENT STIMULANTS ON CALLUSOGENESIS JUGNALIS REGIA EFFIGIA. Izdenister natigeler, [S. l.], n. 2 (98), p. 336–344, 2023. DOI: 10.37884/2-2023/33. Disponível em: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/274. Acesso em: 15 sep. 2026.

“EFFECTS OF DIFFERENT STIMULANTS ON CALLUSOGENESIS JUGNALIS REGIA EFFIGIA”. 2023. Izdenister Natigeler, no. 2 (98) (June): 336-44. https://doi.org/10.37884/2-2023/33.

“EFFECTS OF DIFFERENT STIMULANTS ON CALLUSOGENESIS JUGNALIS REGIA EFFIGIA” (2023) Izdenister natigeler, (2 (98), pp. 336–344. doi:10.37884/2-2023/33.

[1]“EFFECTS OF DIFFERENT STIMULANTS ON CALLUSOGENESIS JUGNALIS REGIA EFFIGIA”, Izdenister natigeler, no. 2 (98), pp. 336–344, Jun. 2023, doi: 10.37884/2-2023/33.

“EFFECTS OF DIFFERENT STIMULANTS ON CALLUSOGENESIS JUGNALIS REGIA EFFIGIA”. Izdenister Natigeler, no. 2 (98), June 2023, pp. 336-44, https://doi.org/10.37884/2-2023/33.

“EFFECTS OF DIFFERENT STIMULANTS ON CALLUSOGENESIS JUGNALIS REGIA EFFIGIA”. Izdenister natigeler, no. 2 (98) (June 29, 2023): 336–344. Accessed September 15, 2026. https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/274.

1.EFFECTS OF DIFFERENT STIMULANTS ON CALLUSOGENESIS JUGNALIS REGIA EFFIGIA. Izdenister natigeler [Internet]. 2023 Jun. 29 [cited 2026 Sep. 15];(2 (98):336-44. Available from: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/274

1.EFFECTS OF DIFFERENT STIMULANTS ON CALLUSOGENESIS JUGNALIS REGIA EFFIGIA. Izdenister natigeler. 2023;(2 (98):336-344. doi:10.37884/2-2023/33

[1] 1. Буркова Е.А., Канарский А.В., Канарская З.А. (2014). Перспектива применения фитобиотехнологии для получения биологически активных веществ //Вестник Казанского технологического университета, 2014. -Т.14. – С.352–356;

[2] 2. Mulabagal V., Tsay H-S. Plant cell cultures -an alternative and efficient source for the production of biologically important secondary metabolites //Int.Journal Applied Science Engineering, 2004. – V.2. - №1. – Р.29-48.

[3] 3. Trandafir I., Cosmulescu S., Botu M., Nour V. Antioxidant activity, and phenolic and mineral contents of the walnut kernel (Juglans regia L.) as a function of the pellicle color //Fruits, 2016. -V. 71. – Р.173–184. doi:10.1051/fruits/2016006.115

[4] 4. Hojatollah Kavosi Ali Khadivi. The selection of superior late-leafing genotypes of Persian walnut (Juglans regia L.) among seedling originated trees based on pomological characterizations //Scientia Horticulturae, 2021. - V.28. -P. 110-299. DOI: 10.1016/j.scienta.2021.110299.

[5] 5. Olimpia A. Iordănescu, Isidora Radulov, Ioana P. Buhan et al. Physical, Nutritional and Functional Properties of Walnuts Genotypes (Juglans regia L.) from Romania //Agronomy, 2021. -V.11. -P.1092. https://doi.org/10.3390/agronomy11061092.

[6] 6. Змушко А.А., Рундя А.П. Размножение грецкого ореха (juglans regia l.) in vitro //Плодоводство, 2015. - Т. 27. – С.404-410.

[7] 7. Ricardo Julian Licea-Moreno, Alexandru Fira, Georgi Chocov. Micropropagation of valuable walnut genotypes for timber production: new advances and insights //Annals of Silvicultural Research, 2020. 44 (1). -P.5-13. DOI: http://dx.doi.org/10.12899/asr-1932.

[8] 8. Кушнаренко С.В., Ромаданова Н.В., Турдиев Т.Т., Аралбаева М.М., Қалыбаев Қ.Р. Сохранение в криобанке образцов грецкого ореха из нескольких популяций сайрам-угамского государственного национального природного парка //Вестник КазНУ. Серия экологическая. -№2. -Т.71. - С.72-80. https://doi.org/10.26577/EJE.2022.v71.i2.07.

[9] 9. Kepeneka, K. and Kolağasib, Z. 2016. Micropropagation of walnut (Juglans regia L.). Acta Physica Polonica, Vol. 130, No. 1, 150 – 156.

[10] 10. López, J.M. Field behavior of self-rooted walnut trees of different cultivars produced by tissue culture and planted in Murcia (Spain) //Acta Hort., 2001.- 544. -P. 543–546.

[11] 11. Manjkhola, S., U. Dhar, and M. Joshi. Organogenesis, embryogenesis and synthetic seed production in Arnebia euchroma an critically endangered medicinal plant of the Himalaya //In Vitro Cell. Dev. Biol.-Plant, 2005. -V.41. – P.244-248.

[12] 12. Bandyopadhyay S., Cane K., Rasmussen G., and Hamill J. Efficient plant regeneration from seedling explants of two commercially important temperate eucalypt species–Eucalyptus nitens and E. Globulus //Plant Sci., 2005. – 140. – P.189-198.

[13] 13. Xiao-Dong CAI, Gui-Yuan WANG, Wen-Juan CAO. In vitro Induction and Proliferation of Callus from Immature Cotyledons and Embryos of Juglans regia cv. ‘Xiangling’ //NotBotHorti Agrobo, 2013. - 41(2). -P.378-384.

[14] 14. Fatima, A., Kamili A.N., and Shah A.M. Plantlet regeneration from excised embryonal axes, shoot apices and nodal segments of Juglans regia L. //Acta Hort., 2006 (ISHS)-705. -P.387-392.

[15] 15. Hasey et al., Hasey, J.K., Westerdahl, B.B., Micke, W.C., Ramos, D.E. &Yeater, J.T. Yield performance of own-rooted “Chandler” walnut versus “Chandler” walnut on Paradox Rootstock //Acta Hort., 2001. -P. 489–493.

[16] 16. Fernández H., Pérez C., and Sánchez-Tamés R. Modulation of the morphogenic potential of the embryonic axis of Juglans regia by cultural conditions //Plant Growth Regul.,2000. -V. 30. -P.125-131.

[17] 17. Vahdati K., Jariteh M., Niknam V., Mirmasoumi M., and Ebrahimzadeh H. Somatic embryogenesis and embryo maturation in Persian walnut //Acta Hort., 2006.- 705. -P.199-205.