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STOCK-RAISING AND VETERINARY

DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK

Bauyrzhan Otarbayev 1 , Madina Sadykanova 1

1 NJSC "Kazakh National Agrarian Research University"

doi.org/10.37884/4-2025/05 pp. 39-48 Admitted 28.08.2025 Published 30.12.2025

Abstract

 

As a result of the study, the activity of conglutinating blood serum in the Sayduldin reaction in interaction with goat's milk was determined, and brucellosis in milk was diagnosed using standard solutions of brucellosis antigen at a concentration of 1.2 AU. The results of the milk study were compared with the approved methods for diagnosing brucellosis using blood serum. According to the results of RBP and RSC, positive results were obtained from 14 blood serum samples. The same results were confirmed in the Saiduldine (MS) reaction, with an additional 6 positive samples identified. In the study of milk in the Saiduldin reaction, the total number of positive samples was 12. These results were confirmed by blood serum testing methods.

According to the results of statistical processing, the average antibody titer in CBD was 1:16 (+15,7%; -13,5%), in MS for blood serum — 1:46 (+26,6%; -21,1%), and in the RS for milk — 1:16 (+15,7%; -13,5%). The difference between the average titers of antibodies in RSC and MS in milk is statistically unreliable, whereas the average titer of antibodies in MS in blood serum is about three times higher than the two above-mentioned tests. In the course of the study, it was proved that the proposed method for diagnosing brucellosis in goat's milk using the Saiduldin reaction is comparable in diagnostic sensitivity to the used methods of blood serum testing — RBP and RSC, but inferior in sensitivity to the Saiduldin reaction with blood serum.

Based on the results obtained, it can be concluded that diagnostically specific antibodies to brucellosis can be detected without taking blood serum from goats, using only milk in the Saiduldin reaction based on conglutination of sheep erythrocytes sensitized by hemolysin under the influence of brucellosis antigen and conglutinating blood serum.

Saiduldin test goat's milk blood serum brucellosis conglutinating blood serum antigen conglutination

01 Introduction

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02 References

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Citation Links

[1]2025. DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK. Izdenister natigeler. 4 (108) (Dec. 2025), 39–48. DOI:https://doi.org/10.37884/4-2025/05.
(1)DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK. Izdenister natigeler 2025, No. 4 (108), 39-48. https://doi.org/10.37884/4-2025/05.
DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK. (2025). Izdenister Natigeler, 4 (108), 39-48. https://doi.org/10.37884/4-2025/05
DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK. Izdenister natigeler, [S. l.], n. 4 (108), p. 39–48, 2025. DOI: 10.37884/4-2025/05. Disponível em: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/1098. Acesso em: 15 sep. 2026.
“DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK”. 2025. Izdenister Natigeler, no. 4 (108) (December): 39-48. https://doi.org/10.37884/4-2025/05.
“DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK” (2025) Izdenister natigeler, (4 (108), pp. 39–48. doi:10.37884/4-2025/05.
[1]“DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK”, Izdenister natigeler, no. 4 (108), pp. 39–48, Dec. 2025, doi: 10.37884/4-2025/05.
“DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK”. Izdenister Natigeler, no. 4 (108), Dec. 2025, pp. 39-48, https://doi.org/10.37884/4-2025/05.
“DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK”. Izdenister natigeler, no. 4 (108) (December 30, 2025): 39–48. Accessed September 15, 2026. https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/1098.
1.DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK. Izdenister natigeler [Internet]. 2025 Dec. 30 [cited 2026 Sep. 15];(4 (108):39-48. Available from: https://kazvetjournal.kaznaru.edu.kz/index.php/research/article/view/1098
1.DIAGNOSIS OF BRUCELLOSIS USING THE SAIDULLDIN REACTION WITH GOAT MILK. Izdenister natigeler. 2025;(4 (108):39-48. doi:10.37884/4-2025/05